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GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody [HSP90B1/1192]

In Stock
Catalog Number Formulation Size Price
7184-RTM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7184-RTM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7184-RTM2-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (HSP90B1/1192).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Properties & Targets

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Hypoxia, Immunology, Cytokine Signaling
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Endoplasmic reticulum lumen, Melanosome, Sarcoplasmic reticulum lumen
Gene Name
HSP90B1
Positive Control
U20S cells. Breast Carcinoma.
Immunogen
Recombinant full-length human HSP90B1 protein
Alternate Names
Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Database Links

Entrez Gene ID
7184
SwissProt
P14625

Additional Information

Clone
HSP90B1/1192
Chromosome Location
12q23.3
Mol. Weight of Antigen
94kDa

Related Antibodies

View All HSP90B1 Antibodies

Functions

  • Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).

Key References

  • Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).
  • Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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