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Printable Datasheet

HA Tag Antibody [16.43]

In Stock
Catalog Number Formulation Size Price
MSM1-2543-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM1-2543-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM1-2543-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Western Blot Analysis of HA-Tag recombinant protein using HA-Tag Mouse Monoclonal Antibody (16.43).

Western Blot Analysis of HA-Tag recombinant protein using HA-Tag Mouse Monoclonal Antibody (16.43).

IF Analysis of transfected PFA-fixed MCF7 cells labeling HA-recombinant protein. HA-Tag Mouse Monoclonal Antibody (16.43) followed by GAM IgG-CF488. Counterstained with RedDot.

IF Analysis of transfected PFA-fixed MCF7 cells labeling HA-recombinant protein. HA-Tag Mouse Monoclonal Antibody (16.43) followed by GAM IgG-CF488. Counterstained with RedDot.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Species Independent
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Any protein with a C-terminal HA tag.
Immunogen
HA-molecule corresponding to amino acids 98-106 (YPYDVPDYA).
Alternate Names
N/A, N/A, HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
16.43
Chromosome Location
Not Known
Mol. Weight of Antigen
60kDa

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Aust et al. The Journal of Immunology, 2009, 183: 106 116

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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