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HBEGF / DTR Antibody [HBEGF/9503]

In Stock
Catalog Number Formulation Size Price
1839-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1839-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1839-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HB-EGF Antibody in Immunohistochemistry (IHC (P))

HB-EGF Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human skin stained with HB-EGF Mouse Monoclonal Antibody (HBEGF/9503). Inset: PBS instead of primary antibody; secondary only negative control.

HBEGF Antibody in SDS-PAGE

HBEGF Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified HBEGF Mouse Monoclonal Antibody (HBEGF/9503). Confirmation of Purity and Integrity of Antibody.

HBEGF Antibody in Western Blot (WB)

HBEGF Antibody in Western Blot (WB)

Western Blot Analysis of RAW 264.7 cell lysate using HBEGF Mouse Monoclonal Antibody (HBEGF/9503). Predicted band size: 23kDa / Observed band size: 27kDa

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Enables growth factor activity and heparin binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; and positive regulation of wound healing. Located in cell surface and extracellular space. Implicated in glomerulosclerosis and perinatal necrotizing enterocolitis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cancer, Angiogenesis, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Membrane, Secreted
Gene Name
HBEGF
Positive Control
RAW 264.7
Immunogen
Recombinant fragment (around aa1-208) of human HBEGF protein (exact sequence is proprietary)
Alternate Names
proheparin-binding EGF-like growth factor, diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody; Diphtheria toxin receptor antibody; DT R antibody DT-R antibody; DTR antibody; DTS antibody; DTSF antibody; HB-EGF antibody; HBEGF antibody; HBEGF_HUMAN antibody; HEGFL antibody; Heparin binding EGF like growth factor antibody; Heparin binding epidermal growth factor antibody; Heparin binding epidermal growth factor like growth factor antibody; Heparin-binding EGF-like growth factor antibody; Proheparin binding EGF like growth factor antibody, diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epidermal growth factor

Database Links

Entrez Gene ID
1839
SwissProt
Q99075

Additional Information

Clone
HBEGF/9503
Chromosome Location
5q31.3
Mol. Weight of Antigen
23kDa

Related Antibodies

View All HBEGF Antibodies

Functions

  • May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Key References

  • Yang CC, Chang KW. Eicosanoids and HB-EGF/EGFR in cancer. Cancer Metastasis Rev. 2018;37(2-3):385-395. doi:10.1007/s10555-018-9746-9
  • Hsieh CH, Chou YT, Kuo MH, Tsai HP, Chang JL, Wu CW. A targetable HB-EGF-CITED4 axis controls oncogenesis in lung cancer. Oncogene. 2017;36(21):2946-2956. doi:10.1038/onc.2016.465
  • Yotsumoto F, Fukagawa S, Miyata K, et al. HB-EGF Is a Promising Therapeutic Target for Lung Cancer with Secondary Mutation of EGFRT790M. Anticancer Res. 2017;37(7):3825-3831. doi:10.21873/anticanres.11761
  • Nishikawa K, Asai T, Shigematsu H, et al. Development of anti-HB-EGF immunoliposomes for the treatment of breast cancer. J Control Release. 2012;160(2):274-280. doi:10.1016/j.jconrel.2011.10.010

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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