Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant HBsAg (Hepatitis B surface Ag a determinant) Antibody [HBsAg/7556R]

In Stock
Catalog Number Formulation Size Price
RBM6-7556-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
RBM6-7556-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
RBM6-7556-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant HBsAg (Hepatitis B surface Ag a determinant) Antibody

SDS-PAGE Analysis of Purified HBsAg Recombinant Rabbit Monoclonal Antibody (HBsAG/7666R). Confirmation of Purity and Integrity of Antibody.

Recombinant HBsAg (Hepatitis B surface Ag a determinant) Antibody - Image 2

Formalin-fixed, paraffin-embedded human HBV-infected liver stained with HBsAg Recombinant Rabbit Monoclonal Antibody (HBsAG/7666R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Hepatitis B surface antigen (HBsAg) is a glycoprotein on the surface of hepatitis B virus. �After hepatitis B infection, HBsAg appears as the first viral marker. �HBsAg can be detected in blood, saliva, breast milk, sweat, tears, nasopharyngeal secretions, semen and vaginal secretions of patients 2 to 6 months after infection with HBV. �HBsAg antibodies are mainly used for the diagnosis of hepatitis B virus.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
HBsAg
Positive Control
HBV infected liver.
Immunogen
Recombinant full-length human HBsAg protein
Alternate Names
Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, HBsAg; HBV major surface antigen; HBV surface antigen; Hepatitis B Virus major surface antigen; Major surface antigen; S antibody

Database Links

Entrez Gene ID
2828293
SwissProt
P03138

Additional Information

Clone
HBsAg/7556R
Chromosome Location
N/A
Mol. Weight of Antigen
42kDa

Related Antibodies

View All HBsAg Antibodies

Functions

  • The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
  • The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

Key References

  • Aden, D.P., et al. 1979. Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282: 615-616.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant HBsAg (Hepatitis B surface Ag a determinant) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales