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Recombinant Heat Shock 27kDa Protein 1 Antibody [rHSPB1/7298]

In Stock
Catalog Number Formulation Size Price
3315-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3315-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3315-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with HSP27 Recombinant Mouse Monoclonal Antibody (rHSPB1/7298). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with HSP27 Recombinant Mouse Monoclonal Antibody (rHSPB1/7298). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

HSP27 Antibody in Western Blot (WB)

HSP27 Antibody in Western Blot (WB)

Western Blot Analysis of Liver and MCF7 lysates using HSP27 Mouse Monoclonal Antibody (rHSPB1/7298).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
BBB VCAM-1 Signaling, MAPK Signaling, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm. Nucleus.
Gene Name
HSPB1
Positive Control
MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27 especially those that are ER and/or PgR positive, Human Liver
Immunogen
Recombinant human full-length HSP27 protein
Alternate Names
Heat shock protein beta-1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Database Links

Entrez Gene ID
3315
SwissProt
P04792

Additional Information

Clone
rHSPB1/7298
Chromosome Location
7q11.23
Mol. Weight of Antigen
27kDa

Related Antibodies

View All HSPB1 Antibodies

Functions

  • Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Key References

  • Georgopoulos, C. and Welch, W.J. 1993. Annu. Rev. Cell Biol. 9: 601-634

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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