Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

HER-2 / c-erbB-2 / neu / CD340 Antibody [ERB2/776]

In Stock
Catalog Number Formulation Size Price
2064-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2064-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2064-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of human trypsinized MCF-7 cells using HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometric Analysis of human trypsinized MCF-7 cells using HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

SDS-PAGE Analysis of Purified HER-2 Mouse Monoclonal Antibody (HRB2/776). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified HER-2 Mouse Monoclonal Antibody (HRB2/776). Confirmation of Integrity and Purity of Antibody.

Immunofluorescent staining of MeOH-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Immunofluorescent staining of MeOH-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Immunofluorescent staining of PFA-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Immunofluorescent staining of PFA-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Autophagy, Breast Cancer, Cancer, Cardiovascular, Developmental Biology, AKT Signaling, Bladder Cancer, Infectious Disease, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Early endosome, Nucleus, Perinuclear region
Gene Name
ERBB2
Positive Control
MCF-7, SKBR-3 cells. Breast Cancers.
Immunogen
Recombinant intracellular domain of human HER-2 protein
Alternate Names
Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog

Database Links

Entrez Gene ID
2064
SwissProt
P04626

Additional Information

Clone
ERB2/776
Chromosome Location
17q12
Mol. Weight of Antigen
185kDa

Related Antibodies

View All ERBB2 Antibodies

Functions

  • Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Key References

  • Wright C, et al. British Journal of Cancer. 65: 118-121 (1992).
  • Utrilla JC, et al. Histopathology. 34: 60-65 (1999).
  • Corbett IP, et al. Journal of Pathology. 161: 15-25 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “HER-2 / c-erbB-2 / neu / CD340 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales