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HER-2 / c-erbB-2 / neu / CD340 Antibody [HRB2/258]

In Stock
Catalog Number Formulation Size Price
2064-MSM2-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HER2 Antibody in Immunofluorescence (IF)

HER2 Antibody in Immunofluorescence (IF)

Immunofluorescent staining of MeOH-fixed SLBR3 cells with HER2 Mouse Monoclonal Antibody (HRB2/258) followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

HER-2 Antibody in SDS-PAGE

HER-2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified HER-2 Mouse Monoclonal Antibody (HRB2/258). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human, Rat
Research Areas
Autophagy, Breast Cancer, Cancer, Cardiovascular, Developmental Biology, AKT Signaling, Bladder Cancer, Infectious Disease, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell surface
Gene Name
ERBB2
Positive Control
SKBR-3 cells. Breast Cancers.
Immunogen
Recombinant extracellular domain of human HER-2 protein
Alternate Names
Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog

Database Links

Entrez Gene ID
2064
SwissProt
P04626

Additional Information

Clone
HRB2/258
Chromosome Location
17q12
Mol. Weight of Antigen
185kDa

Related Antibodies

View All ERBB2 Antibodies

Functions

  • Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Key References

  • Wright C, et al. British Journal of Cancer. 65: 118-121 (1992).
  • Utrilla JC, et al. Histopathology. 34: 60-65 (1999).
  • Corbett IP, et al. Journal of Pathology. 161: 15-25 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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