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HSP27 (Heat Shock Protein 27) Antibody [SPM252]

In Stock
Catalog Number Formulation Size Price
3315-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3315-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3315-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HSP27 (Heat Shock Protein 27) Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).

HSP27 (Heat Shock Protein 27) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).

HSP27 (Heat Shock Protein 27) Antibody - Image 3

Western blot analysis of HeLa cell lysate using HSP27 Mouse Monoclonal Antibody (SPM252).

HSP27 (Heat Shock Protein 27) Antibody - Image 4

SDS-PAGE Analysis Purified HSP27 Mouse Monoclonal Antibody (SPM252). Confirmation of Integrity and Purity of Antibody.

HSP27 (Heat Shock Protein 27) Antibody - Image 5

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27. HSP27 Mouse Monoclonal Antibody (SPM252) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.

HSP27 (Heat Shock Protein 27) Antibody - Image 6

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (SPM252) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep
Research Areas
BBB VCAM-1 Signaling, MAPK Signaling, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus, Spindle
Gene Name
HSPB1
Positive Control
especially those that are positive for ER and/or PgR., MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27
Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Alternate Names
Heat shock protein beta-1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Database Links

Entrez Gene ID
3315
SwissProt
P04792

Additional Information

Clone
SPM252
Chromosome Location
7q11.23
Mol. Weight of Antigen
27kDa

Related Antibodies

View All HSPB1 Antibodies

Functions

  • Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Key References

  • Edwards DP et. al.BiochemBiophys Research Commun, 93:804-812, 1980.
  • Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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