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HSP27 (Heat Shock Protein 27) Antibody [HSPB1/774]

In Stock
Catalog Number Formulation Size Price
3315-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3315-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3315-MSM2-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HSP27 (Heat Shock Protein 27) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

HSP27 (Heat Shock Protein 27) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

HSP27 (Heat Shock Protein 27) Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Monoclonal Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

HSP27 (Heat Shock Protein 27) Antibody - Image 4

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 MAb (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

HSP27 (Heat Shock Protein 27) Antibody - Image 5

SDS-PAGE Analysis Purified HSP27 MAb (HSPB1/774). Confirmation of Integrity and Purity of Antibody.

HSP27 (Heat Shock Protein 27) Antibody - Image 6

Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep
Research Areas
BBB VCAM-1 Signaling, MAPK Signaling, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus, Spindle
Gene Name
HSPB1
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR., HeLa or MCF-7 cells
Immunogen
Recombinant human HSPB1 protein
Alternate Names
Heat shock protein beta-1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Database Links

Entrez Gene ID
3315
SwissProt
P04792

Additional Information

Clone
HSPB1/774
Chromosome Location
7q11.23
Mol. Weight of Antigen
27kDa

Related Antibodies

View All HSPB1 Antibodies

Functions

  • Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Key References

  • Georgopoulos, C. and Welch, W.J. 1993. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol. 9: 601-634.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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