Recombinant Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody [235-1R]

Name: Recombinant Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody
SKU: RBM8-8775
Availability: InStock

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SDS-PAGE Analysis of Purified HuNu Recombinant Rabbit Monoclonal Antibody (235-1R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Properties & Targets

Antibody Type

Recombinant Rabbit Monoclonals

Host

Rabbit

Applications

Immunohistochemistry

Species Reactivity

Human

Isotype / Light Chain

IgG / Kappa

Cellular Localization

Nucleus.

Positive Control

All human cells. Human tonsil.

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Alternate Names

, HNA; HuNu

Database Links

Entrez Gene ID

Not Known

SwissProt

Not Known

Additional Information

Clone

235-1R

Chromosome Location

Not Known

Mol. Weight of Antigen

70kDa & 80kDa

Key References

Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008;131(8): 2127-2139.
Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Recombinant Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody [235-1R]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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