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IGF2BP2 Antibody [PCRP-IGF2BP2-1F9]

In Stock
Catalog Number Formulation Size Price
10644-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10644-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10644-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells

Summary

IGF2BP2 (Insulin-like growth factor 2 mRNA binding protein 2) is also known as IGF2 mRNA-binding protein 2, IMP-2 (IGF-II mRNA-binding protein 2), VICKZ family member 2 or hepatocellular carcinoma autoantigen p62 and is a 556 amino acid protein. IGF2BP2 is expressed in a variety of tissues including heart, placenta, skeletal muscle, pancreas, fetal liver, lung, kidney, thymus and gonadal cells. IGF2BP2 is an RNA binding protein which may be involved in the regulation of mRNA translation and may also function to control the spatial localization of target mRNAs. Antibodies against IGF2BP2 have been detected in patients with HCC (hepatocellular carcinoma), suggesting that IGF2BP2 may have a role in the pathogenesis of HCC. Defects in IGF2BP2 are thought to be associated with susceptibility to type two diabetes mellitus.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa cells.
Immunogen
Recombinant full-length human IGF2BP2 protein
Alternate Names
Insulin-like growth factor 2 mRNA-binding protein 2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2, Hepatocellular carcinoma autoantigen p62; IGF 2 mRNA binding protein 2; IGF II mRNA binding protein 2; IMP2; Insulin like growth factor 2 mRNA binding protein 2; p62; VICKZ 2; VICKZ family member 2; VICKZ2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-IGF2BP2-1F9
Chromosome Location
3q27.2
Mol. Weight of Antigen
105kDa

Functions

  • RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed:29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs (PubMed:9891060). Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed:29476152).

Key References

  • Blackshaw S, et al. Nature methods15.5 (2018 May): 330-338.
  • Statement for PublicationAll investigators should include an acknowledgment in the publications the benefit from the use of this product. Following statement is suggested: PCRP-IGF2BP2-1F9 was developed by JHU/CDI under Protein Capture Reagents Program of NICHD of the NIH.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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