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IgG (Immunoglobulin Gamma Heavy Chain) (B Cell Marker) Antibody [SPM556]

Name: IgG (Immunoglobulin Gamma Heavy Chain) (B Cell Marker) Antibody
SKU: 3500-MSM2X
Availability: InStock

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IgG (Immunoglobulin Gamma Heavy Chain) (B Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Monoclonal Antibody (SPM556)

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Isotype / Light Chain

IgG2a / Kappa

Cellular Localization

Cell membrane, Secreted

Gene Name

IGHG1

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen.

Immunogen

Purified human Ig Gamma Chain

Alternate Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

Database Links

Entrez Gene ID

3500; 3501; 3502; 3503

SwissProt

P01857; P01859; P01860; P01861

Additional Information

Clone

SPM556

Chromosome Location

14q32.33

Mol. Weight of Antigen

75kDa

Functions

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Key References

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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IgG (Immunoglobulin Gamma Heavy Chain) (B Cell Marker) Antibody [SPM556]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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