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Insulin-like Growth Factor-1 (IGF-1) Antibody [SPM406]

In Stock
Catalog Number Formulation Size Price
3479-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3479-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3479-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse, Rabbit, Rat
Research Areas
Breast Cancer, Cardiovascular, AKT Signaling, Mesenchymal Stem Cell Differentiation, Neural Stem Cells, Neuroinflammation, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
IGF1
Positive Control
Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein.
Immunogen
Purified human IGF-1 protein
Alternate Names
Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, Insulin-like Growth Factor IA (IGF-IA); Insulin-like Growth Factor IB (IGF-IB); Mechano Growth Factor (MGF); Somatomedin-C

Database Links

Entrez Gene ID
3479
SwissProt
P05019

Additional Information

Clone
SPM406
Chromosome Location
12q23.2
Mol. Weight of Antigen
~7.6kDa

Related Antibodies

View All IGF1 Antibodies

Functions

  • The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).

Key References

  • Sandberg-Nordqvist AC, et. al. (1993) Cancer Res. 53: 2475-2478.
  • Rotwein p, et. al. (1986) J. Biol. Chem. 261: 4828-4832.
  • Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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