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Interleukin 10 (IL10) (Immuno-regulation Marker) Antibody [IL10/8711]

In Stock
Catalog Number Formulation Size Price
3586-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3586-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3586-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate cancer stained with IL10 Mouse Monoclonal Antibody (IL10/8711). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human prostate cancer stained with IL10 Mouse Monoclonal Antibody (IL10/8711). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified IL10 Mouse Monoclonal Antibody (IL10/8711). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified IL10 Mouse Monoclonal Antibody (IL10/8711). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Interleukin 10 (IL10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immuno-regulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immuno-regulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Hematopoietic Stem Cells, Infectious Disease, Neuroinflammation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm (secreted). Cell Surface.
Gene Name
IL10
Positive Control
Human adrenal or lymph node tissues (IHC).
Immunogen
Recombinant full-length human Interleukin 10 (IL10) protein
Alternate Names
Interleukin-10, Cytokine synthesis inhibitory factor, Cytokine Synthesis Inhibitory Factor (CSIF); GVHDS; IL-10; IL10; IL10A; Interleukin-10; T-cell Growth Inhibitory Factor (TGIF)

Database Links

Entrez Gene ID
3586
SwissProt
P22301

Additional Information

Clone
IL10/8711
Chromosome Location
1q32.1
Mol. Weight of Antigen
20kDa (Monomer); 37kDa (Dimer)

Related Antibodies

View All IL10 Antibodies

Functions

  • Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Key References

  • Bromberg, J.S. 1995. IL-10 immunosuppression in transplantation. Curr. Opin. Immunol. 7: 639-643.
  • Fleming, S.D. and Campbell, P.A. 1996. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J. Immunol. 156: 1143-1150.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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