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Lambda Light Chain (B-Cell Marker) Antibody [LcN-2]

In Stock
Catalog Number Formulation Size Price
3535-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3535-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3535-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Lambda Light Chain Antibody in Immunofluorescence (IF)

Lambda Light Chain Antibody in Immunofluorescence (IF)

Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Lambda Light Chain Monoclonal Antibody (LcN-2) (green). DAPI was used to stain the cell nuclei (blue).

Isotype Control Antibody in Immunofluorescence (IF)

Isotype Control Antibody in Immunofluorescence (IF)

Confocal Immunofluorescent analysis of Ramos cells using CF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Lambda Light Chain Antibody in Immunohistochemistry (IHC (P))

Lambda Light Chain Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Tonsil stained with Lambda Light Chain Monoclonal Antibody (LcN-2).

Lambda Light Chain Antibody in Western Blot (WB)

Lambda Light Chain Antibody in Western Blot (WB)

Western blot analysis of human Intestinal tissue lysate using Lambda Light Chain Monoclonal Antibody (LcN-2).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
IGLV1-51
Positive Control
293T, Raji or hPBL cells. Human tonsil or spleen.
Immunogen
Purified human IgG
Alternate Names
Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein

Database Links

Entrez Gene ID
3535; 3546
SwissProt
P01701; P01842

Additional Information

Clone
LcN-2
Chromosome Location
22q11.22
Mol. Weight of Antigen
~22.5kDa

Related Antibodies

View All IGLV1-51 Antibodies

Functions

  • V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Key References

  • Campbell JP et. al. J Immunol Methods. 2013;391(1-2):1-13.

PubMed Links

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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