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Langerin / CD207 (Marker of Langerhans Cells) Antibody [LGRN/7356]

In Stock
Catalog Number Formulation Size Price
50489-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
50489-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
50489-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified Langerin Mouse Monoclonal Antibody (LGRN/7356). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Langerin Mouse Monoclonal Antibody (LGRN/7356). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with Langerin Mouse Monoclonal Antibody (LGRN/7356). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human skin stained with Langerin Mouse Monoclonal Antibody (LGRN/7356). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia. Epidermal LCs possess strong immuno-stimulatory capacity and play a central role in the initiation and regulation of immune responses. Langerin (CD207) is a Ca2+-dependent, C-type lectin domain containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG). BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T cells. Human spleen, lymph node, thymus, liver, lung and heart express Langerin protein.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Dendritic Cell Marker
Isotype / Light Chain
IgG /
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
CD207
Positive Control
Human skin.
Immunogen
Recombinant fragment (around aa50-250) of human Langerin protein (exact sequence is proprietary)
Alternate Names
C-type lectin domain family 4 member K, Langerin, C-type lectin domain family 4 member K; CD207; CLEC4K; Langerhans cell specific c type lectin; Langerin

Database Links

Entrez Gene ID
50489
SwissProt
Q9UJ71

Additional Information

Clone
LGRN/7356
Chromosome Location
2p13.3
Mol. Weight of Antigen
48kDa

Related Antibodies

View All CD207 Antibodies

Functions

  • Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.

Key References

  • Valladeau, J., et al. 2000. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 12: 71-81.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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