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Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody [rMLANA/8180]

In Stock
Catalog Number Formulation Size Price
2315-MSM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2315-MSM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2315-MSM22-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human skin stained with MART-1 Recombinant Mouse Monoclonal Antibody (rMLANA/8180). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

MART-1 Antibody in Western Blot (WB)

MART-1 Antibody in Western Blot (WB)

Western Blot Analysis of Colo-38 lysate using MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/8180).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm.
Gene Name
MLANA
Positive Control
SK-MEL-13 or SK-MEL-19 melanoma cell lines. Melanomas, Colo-38 cells
Immunogen
Recombinant full-length human MART-1 protein
Alternate Names
Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

Database Links

Entrez Gene ID
2315
SwissProt
Q16655Human

Additional Information

Clone
rMLANA/8180
Chromosome Location
9p24.1
Mol. Weight of Antigen
20-22kDa (doublet)

Related Antibodies

View All MLANA Antibodies

Functions

  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Key References

  • Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
  • Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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