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MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146 Antibody [C146/634]

In Stock
Catalog Number Formulation Size Price
4162-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4162-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4162-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Melanoma stained with CD146 Monoclonal Antibody (C146/634)

Formalin-fixed, paraffin-embedded human Melanoma stained with CD146 Monoclonal Antibody (C146/634)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD146 Monoclonal Antibody (C146/634)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD146 Monoclonal Antibody (C146/634)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Endothelial Cell Marker, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
MCAM
Positive Control
A-375, HUV-EC or HeLa cells. Tonsil or Melanoma (IHC).
Immunogen
Recombinant human CD146 protein
Alternate Names
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

Database Links

Entrez Gene ID
4162
SwissProt
P43121

Additional Information

Clone
C146/634
Chromosome Location
11q23.3
Mol. Weight of Antigen
130kDa

Related Antibodies

View All MCAM Antibodies

Functions

  • Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Key References

  • Pruszak J et al. Stem Cells 25:2257-68 (2007)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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