Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant MCM3 (Proliferation Marker) Antibody [MCM3/8972R]

In Stock
Catalog Number Formulation Size Price
4172-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4172-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4172-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with MCM3 Recombinant Rabbit Monoclonal Antibody (MCM3/8972R). Inset: PBS instead of primary antibody, secondary only control.

Formalin-fixed, paraffin-embedded human tonsil stained with MCM3 Recombinant Rabbit Monoclonal Antibody (MCM3/8972R). Inset: PBS instead of primary antibody, secondary only control.

SDS-PAGE Analysis of Purified MCM3 Recombinant Rabbit Monoclonal Antibody (MCM3/8972R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified MCM3 Recombinant Rabbit Monoclonal Antibody (MCM3/8972R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
MCM3
Positive Control
Jurkat or Ramos cells. Human colon or tonsil.|MCF-7
Immunogen
Recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary)
Alternate Names
DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, Cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta

Database Links

Entrez Gene ID
4172
SwissProt
P25205

Additional Information

Clone
MCM3/8972R
Chromosome Location
6p12.2
Mol. Weight of Antigen
115kDa

Related Antibodies

View All MCM3 Antibodies

Functions

  • Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (Probable).

Key References

  • Koonin, E.V. 1993. Nucleic Acids Res. 21: 2541-2547.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant MCM3 (Proliferation Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales