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SDS-PAGE Analysis of Purified MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using MED7-Monospecific Mouse Monoclonal Antibody (PCRP-MED7-1B8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
MED7, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.
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