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Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45]

In Stock
Catalog Number Formulation Size Price
MSM2-702-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-702-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-702-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody - Image 2

Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1, IgG2a / Kappa
Cellular Localization
Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane
Gene Name
MLANA
Positive Control
COLO-38 or SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Immunogen
Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Alternate Names
Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17

Database Links

Entrez Gene ID
2315; 7299; 6490
SwissProt
Q16655; P14679; P40967

Additional Information

Clone
A103 + T311 + HMB45
Mol. Weight of Antigen
20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 26-100kDa (gp100)

Related Antibodies

View All MLANA Antibodies

Functions

  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Key References

  • Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
  • Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
  • Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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