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MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Antibody [MMP2/4586]

In Stock
Catalog Number Formulation Size Price
4313-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4313-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4313-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Antibody

SDS-PAGE Analysis of Purified MMP2 Mouse Monoclonal Antibody (MMP2/4586). Confirmation of Integrity and Purity of Antibody.

MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human placenta stained with MMP2 Mouse Monoclonal Antibody (MMP2/4586). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Immunology, Angiogenesis, BBB VCAM-1 Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm. Cell surface.
Gene Name
MMP2
Positive Control
U-138 MG or U-87 MG cells. Human placenta or colon carcinoma.
Immunogen
Recombinant human MMP2 protein (exact sequence is proprietary)
Alternate Names
72 kDa type IV collagenase | PEX, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, 72kD type IV collagenase; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metalloproteinase-2; MMP2; MONA; Neutrophil gelatinase; PEX; TBE-1

Database Links

Entrez Gene ID
4313
SwissProt
P08253

Additional Information

Clone
MMP2/4586
Chromosome Location
16q12.2
Mol. Weight of Antigen
72kDa (Pro); 63kDa (cleaved)

Related Antibodies

View All MMP2 Antibodies

Functions

  • Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
  • -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
  • PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Key References

  • Huhtala, P., et al. 1990. Structure of the human type IV collagenase gene. J. Biol. Chem. 265: 11077-11082.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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