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Moesin Antibody [MSN/491]

In Stock
Catalog Number Formulation Size Price
4478-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4478-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4478-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Moesin Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 2

Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 3

Formalin-fixed, paraffin-embedded Rat Lung stained with Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 4

Formalin-fixed, paraffin-embedded Rat Colon stained with Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 5

Western blot of HT29 cell lysate using Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 6

SDS-PAGE Analysis Purified Moesin Mouse Monoclonal Antibody (MSN/491).

Moesin Antibody - Image 7

Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Mouse Monoclonal Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Moesin Antibody - Image 8

Flow Cytometric Analysis of K562 cells using Moesin Mouse Monoclonal Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Moesin Antibody - Image 9

Western Blot Analysis of human PC3 cell lysate using Mouse Monoclonal Antibody (MSN/491).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Research Areas
Autophagy, Cancer, Developmental Biology, Immunology, BBB VCAM-1 Signaling, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Microvillus, Microvillus membrane
Gene Name
MSN
Positive Control
CH3LC or HUVEC cells. Uterus, HT-29, K562, Melanoma, Placenta, Testicular Carcinoma., Tonsil
Immunogen
Recombinant full-length human Moesin protein
Alternate Names
Moesin, Membrane-organizing extension spike protein, Membrane-organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN/ALK fusion

Database Links

Entrez Gene ID
4478
SwissProt
P26038

Additional Information

Clone
MSN/491
Chromosome Location
Xq11.1
Mol. Weight of Antigen
78kDa

Related Antibodies

View All MSN Antibodies

Functions

  • Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton (PubMed:10212266). Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement (PubMed:10212266). These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction (PubMed:12387735, PubMed:15039356). The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs (PubMed:9298994, PubMed:9616160). Modulates phagolysosomal biogenesis in macrophages (By similarity). Participates also in immunologic synapse formation (PubMed:27405666).

Key References

  • Lankes W et. al., Biochem Journal, 1988; 251:831-842.
  • Schwartz-Albiez R et. al., European Journal Cell Biology, 1995; 67:189-198.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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