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MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [Mc5]

In Stock
Catalog Number Formulation Size Price
4582-MSM29-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4582-MSM29-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4582-MSM29-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human stomach stained with MUC1 Mouse Recombinant Monoclonal Antibody (Mc5). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human stomach stained with MUC1 Mouse Recombinant Monoclonal Antibody (Mc5). Inset: PBS instead of primary antibody, secondary negative control.

SDS-PAGE Analysis of Purified MUC1 Mouse Recombinant Monoclonal Antibody (Mc5). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified MUC1 Mouse Recombinant Monoclonal Antibody (Mc5). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The mucin antigen recognized by Mc-5 is a glycosylated molecule with a molecular mass of 400 kD. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Staining, however, is the strongest in mammary epithelia. The combination of positive staining for keratin with negative EMA can be used to phenotype the above-mentioned epithelial tumors. Recognizes the mucin antigen that is present in most epithelial tissues (breast, colonic, bronchial, gastric, etc.) This antibody does not recognize myelopoietic, lymphopoietic, fibroblasts or sarcomatous cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Human breast carcinoma or normal breast.
Immunogen
Delipidated human milk fat globules
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Database Links

Entrez Gene ID
4582
SwissProt
P15941

Additional Information

Clone
Mc5
Chromosome Location
1q22
Mol. Weight of Antigen
>400kDa

Related Antibodies

View All MUC1 Antibodies

Functions

  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
  • The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Key References

  • Peterson, JA, et al. Hybridoma 9:221-235, 1990.
  • Gendler, S, et al. J Biol Chem 263:12820-12823, 1988.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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