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Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [MUC1/4416R]

In Stock
Catalog Number Formulation Size Price
4582-RBM26-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4582-RBM26-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4582-RBM26-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Western blot analysis of MCF-7 cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Western blot analysis of MCF-7 cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

SDS-PAGE Analysis of Purified MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R). Confirmation of Purity and Integrity of Antibody.

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of T47D cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Colon, MCF-7 or MDA-231 cells. Human breast, ovarian or endometrial carcinoma.
Immunogen
Recombinant full-length human MUC1 protein
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Database Links

Entrez Gene ID
4582
SwissProt
P15941

Additional Information

Clone
MUC1/4416R
Chromosome Location
1q22
Mol. Weight of Antigen
265-400kDa

Related Antibodies

View All MUC1 Antibodies

Functions

  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
  • The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Key References

  • Cordell J et al. 1985. Br J Cancer 52(3):347-54.
  • Heyderman E et al. 1985. Br J Cancer 52(3):355-61.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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