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Recombinant Myelin Basic Protein Antibody [rMBP/9301]

In Stock
Catalog Number Formulation Size Price
4155-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4155-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4155-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE AnalysisPurified MBP Recombinant Mouse Monoclonal Antibody (rMBP/9301).Confirmation of Purity and Integrity of Antibody.

SDS-PAGE AnalysisPurified MBP Recombinant Mouse Monoclonal Antibody (rMBP/9301).Confirmation of Purity and Integrity of Antibody.

MBP Antibody in Western Blot (WB)

MBP Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/9301).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. rMBP/9301 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. rMBP/9301 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Neuroscience, Complement System
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Myelin membrane; Peripheral membrane protein; Cytoplasmic side (of myelin).
Gene Name
MBP
Positive Control
Human brain or astrocytoma tissue.
Immunogen
Recombinant fragment (around aa1-157) of human MBP protein (exact sequence is proprietary)
Alternate Names
Myelin basic protein, Myelin A1 protein, Myelin membrane encephalitogenic protein, GDB; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MLD; Myelin A1 Protein, basic; Myelin Deficient; Myelin membrane encephalitogenic protein; SHI; Shiverer; SP

Database Links

Entrez Gene ID
4155
SwissProt
P02686

Additional Information

Clone
rMBP/9301
Chromosome Location
18q23
Mol. Weight of Antigen
13-21kDa

Related Antibodies

View All MBP Antibodies

Functions

  • The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

Key References

  • Matsuo, A., et al. (1997). The American Journal of Pathology, 150(4), 1253-1266.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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