Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

MyoD1 (Rhabdomyosarcoma Marker) Antibody [5.8A]

In Stock
Best Seller
Catalog Number Formulation Size Price
4654-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4654-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4654-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
MyoD1 (Rhabdomyosarcoma Marker) Antibody

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (5.8).

MyoD1 (Rhabdomyosarcoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified MyoD1 Mouse Monoclonal Antibody (5.8A). Confirmation of Integrity and Purity of Antibody.

MyoD1 (Rhabdomyosarcoma Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (5.8).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Chicken, Human, Mouse, Rat
Research Areas
Cardiovascular, Developmental Biology, Mesenchymal Stem Cell Differentiation, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
MYOD1
Positive Control
Rhabdomyosarcoma
Immunogen
Recombinant mouse MyoD1 protein
Alternate Names
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Database Links

Entrez Gene ID
4654
SwissProt
P15172

Additional Information

Clone
5.8A
Chromosome Location
11p15.1
Mol. Weight of Antigen
45kDa

Related Antibodies

View All MYOD1 Antibodies

Functions

  • Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

Key References

  • Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
  • Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
  • Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “MyoD1 (Rhabdomyosarcoma Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales