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MyoD1 (Rhabdomyosarcoma Marker) Antibody [SPM427]

In Stock
Catalog Number Formulation Size Price
4654-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4654-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4654-MSM1X-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (SPM427).

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (SPM427).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Chicken, Human, Mouse, Rat
Research Areas
Autophagy, Cancer, Cardiovascular, Developmental Biology, Mesenchymal Stem Cell Differentiation, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
MYOD1
Positive Control
Rhabdomyosarcoma.
Immunogen
Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
Alternate Names
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Database Links

Entrez Gene ID
4654
SwissProt
P15172

Additional Information

Clone
SPM427
Chromosome Location
11p15.1
Mol. Weight of Antigen
45kDa

Related Antibodies

View All MYOD1 Antibodies

Functions

  • Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

Key References

  • Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
  • Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
  • Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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