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N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody [CDH2/9489]

In Stock
Catalog Number Formulation Size Price
1000-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1000-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1000-MSM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
N-Cadherin Antibody in Western Blot (WB)

N-Cadherin Antibody in Western Blot (WB)

Western Blot Analysis of human heart tissue lysate using N-Cadherin Mouse Monoclonal Antibody (CDH2/9489).

N-Cadherin Antibody in SDS-PAGE

N-Cadherin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified N-Cadherin Mouse Monoclonal Antibody (CDH2/9489). Confirmation of Purity and Integrity of Antibody.

N-Cadherin Antibody in Immunohistochemistry (IHC (P))

N-Cadherin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human heart muscle stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/9489). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Cell surface, Sarcolemma
Gene Name
CDH2
Positive Control
HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex.
Immunogen
Recombinant fragment (around aa400-600) of human CDH2 protein (exact sequence is proprietary)
Alternate Names
Cadherin-2, CDw325, Neural cadherin, Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD

Database Links

Entrez Gene ID
1000
SwissProt
P19022

Additional Information

Clone
CDH2/9489
Chromosome Location
18q11.2
Mol. Weight of Antigen
130-140kDa

Related Antibodies

View All CDH2 Antibodies

Functions

  • Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Key References

  • Wahl, JK III; Kim, YJ; Cullen, JM; Johnson, KR; Wheelock, MJ.N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003, 278(19):17269-76

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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