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Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody [NAPSA/1865R]

In Stock
Catalog Number Formulation Size Price
9476-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
9476-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
9476-RBM5-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

SDS-PAGE Analysis of Purified Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

SDS-PAGE Analysis of Purified Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

Napsin-A Antibody in Western blot (WB)

Napsin-A Antibody in Western blot (WB)

Western Blot Analysis of Human Kidney lysate using Napsin-A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Dendritic Cell Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
NAPSA
Positive Control
Lung adenocarcinoma, Human Kidney
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Alternate Names
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Database Links

Entrez Gene ID
9476
SwissProt
O96009

Additional Information

Clone
NAPSA/1865R
Chromosome Location
19q13.33
Mol. Weight of Antigen
37kDa

Related Antibodies

View All NAPSA Antibodies

Functions

  • May be involved in processing of pneumocyte surfactant precursors.

Key References

  • Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  • Bishop, JA et. al. Hum Pathol 41: 20-25
  • Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-317

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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