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Nuclear Mitotic Apparatus Protein (NuMA) Antibody [A73-B/D12]

In Stock
Catalog Number Formulation Size Price
4926-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4926-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4926-MSM1-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Nuclear Mitotic Apparatus Protein (NuMA) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with NuMA Monoclonal Antibody (A73-B/D12)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cancer, Nuclear Marker
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Cell cortex, Cell membrane, Centrosome, Chromosome, Cytoplasm, Cytoskeleton, Cytosol, Lateral cell membrane, Microtubule organizing center, Nucleoplasm, Nucleus, Nucleus matrix, Spindle pole
Gene Name
NUMA1
Positive Control
Exponentially growing any cultured human cells. Tonsil or lymph node.
Immunogen
Colon carcinoma 174T cells
Alternate Names
Nuclear mitotic apparatus protein 1, Nuclear matrix protein-22, Nuclear mitotic apparatus protein, SP-H antigen, Nuclear Mitotic Apparatus Protein 1; NuMA protein; NUMA1; SP-H antigen; Structural nuclear protein;

Database Links

Entrez Gene ID
4926
SwissProt
Q14980

Additional Information

Clone
A73-B/D12
Chromosome Location
11q13
Mol. Weight of Antigen
228kDa

Related Antibodies

View All NUMA1 Antibodies

Functions

  • Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24996901, PubMed:24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24996901, PubMed:24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348). Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386). Involved in anastral spindle assembly (PubMed:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).

Key References

  • Compton DA and Cleveland DW. NuMA is required for the proper completion of mitosis. J Cell Biol 1993, 120(4):947-57
  • Price CM and Pettijohn DE. Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of Purified NuMA protein. Exp Cell Res 1986, 166:295-311.
  • Butschak G et al. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem 1995, 97(1):19-31
  • Gueth-Hallonet C et al. Cleavage of the nuclear matrix protein NuMA during apoptosis. Exp Cell Res 1997, 233(1):21-24.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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