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Recombinant Nucleolin (Marker of Human Cells) Antibody [NCL/7014R]

In Stock
Catalog Number Formulation Size Price
4691-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4691-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4691-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Western blot analysis of Jurkat cell lysate using Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Western blot analysis of Jurkat cell lysate using Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).

Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleolus, Nucleus
Gene Name
NCL
Positive Control
All human cells. Human testis, ovary or Hodgkin s lymphoma.
Immunogen
Recombinant full-length human NCL protein
Alternate Names
Nucleolin, Protein C23, NCL; Nucl; Nucleolin; Protein C23

Database Links

Entrez Gene ID
4691
SwissProt
P19338

Additional Information

Clone
NCL/7014R
Chromosome Location
2q37.1
Mol. Weight of Antigen
76kDa

Related Antibodies

View All NCL Antibodies

Functions

  • Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Key References

  • Qiu W,Zhou F,Zhang Q,Sun X,Shi X,Liang Y,Wang X,Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS.2013;121(10):919-25.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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