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Recombinant Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody [NPM1/7072R]

In Stock
Catalog Number Formulation Size Price
4869-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4869-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4869-RBM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with NucleophosminRecombinant Rabbit Monoclonal Antibody (NPM1/7072R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody - Image 2

SDS-PAGE Analysis Purified NPM1Recombinant Rabbit Monoclonal Antibody (NPM1/7072R). Confirmation of Integrity and Purity of Antibody.

Recombinant Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified NPM1Recombinant Rabbit Monoclonal Antibody (NPM1/7072R). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm.It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Infectious Disease, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleolus, Nucleoplasm, Nucleus
Gene Name
NPM1
Positive Control
HeLa or A431 cells. Human skin or colon tissue (IHC)., K562, MCF-7
Immunogen
Recombinant fragment of human NPM1 protein (exact sequence is proprietary)
Alternate Names
Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NO38; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; Nucleophosmin/nucleoplasmin family member 1; Numatrin

Database Links

Entrez Gene ID
4869
SwissProt
P06748

Additional Information

Clone
NPM1/7072R
Chromosome Location
5q35.1
Mol. Weight of Antigen
33kDa

Related Antibodies

View All NPM1 Antibodies

Functions

  • Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).

Key References

  • Nozawa, Y., et al. 1996. Expression of nucleophosmin/B23 in normal and neoplastic colorectal mucosa. J. Pathol. 178: 48-52.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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