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Recombinant OCT-2 (POU2F2) (B-Cell Marker) Antibody [OCT2/7073R]

In Stock
Catalog Number Formulation Size Price
5452-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5452-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5452-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with Oct-2 Recombinant Mouse Monoclonal Antibody (rOCT2/7196). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with Oct-2 Recombinant Mouse Monoclonal Antibody (rOCT2/7196). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with Oct-2 Recombinant Mouse Monoclonal Antibody (rOCT2/7196). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with Oct-2 Recombinant Mouse Monoclonal Antibody (rOCT2/7196). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. Oct-2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of Oct-2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of Oct-2. Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for Oct-2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
POU2F2
Positive Control
Raji or U-937 cells. Human spleen or tonsil.
Immunogen
Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)
Alternate Names
POU domain, class 2, transcription factor 2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; POU domain class 2 transcription factor 2; POU2F2

Database Links

Entrez Gene ID
5452
SwissProt
P09086

Additional Information

Clone
OCT2/7073R
Chromosome Location
19q13.2
Mol. Weight of Antigen
60kDa

Related Antibodies

View All POU2F2 Antibodies

Functions

  • Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).
  • Activates the U2 small nuclear RNA (snRNA) promoter.

Key References

  • Gibson SE, et al. Am J Clin Pathol. 2006; 126:916-24.
  • Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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