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Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody [OCT4/6875R]

In Stock
Catalog Number Formulation Size Price
5460-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5460-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5460-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
POU5F1
Positive Control
Raji or U-937 cells. Human seminoma or testicular carcinoma
Immunogen
Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)
Alternate Names
POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, OCT3; OCT3A; OCT4; OCT4A; OCT-3/4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Database Links

Entrez Gene ID
5460
SwissProt
Q01860

Additional Information

Clone
OCT4/6875R
Chromosome Location
6p21.33
Mol. Weight of Antigen
38.6kDa

Related Antibodies

View All POU5F1 Antibodies

Functions

  • Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Key References

  • Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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