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Recombinant P-glycoprotein / p170 / MDR1 / CD243 Antibody [MDR1/8986R]

In Stock
Catalog Number Formulation Size Price
5243-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5243-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5243-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human adrenal gland stained with MDR1 Recombinant Rabbit Monoclonal Antibody (MDR1/8986R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human adrenal gland stained with MDR1 Recombinant Rabbit Monoclonal Antibody (MDR1/8986R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cells selected for resistance to a single cytotoxic drug may become crossresistant to a broad range of drugs with different structures and cellular targets. This phenomenon is called multiple drug resistance (MDR). MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. The Mdr-1 gene is known as ABCB1 and is located on human chromosome 7q21.12. The mouse homolog of Mdr-1 is known as Mdr-3. Interestingly, a murine protein by the name of Mdr-1 exists and is encoded by the murine Abcb1b gene, but it is not homologous with human Mdr-1.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane.
Gene Name
ABCB1
Positive Control
hPBL or Jurkat whole cell lysate. Human adrenal gland.
Immunogen
Recombinant fragment (around aa500-700) of human ABCB1 protein (exact sequence is proprietary)
Alternate Names
ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1, ABC20; ABCB1; ATP-binding cassette sub-family B member 1; CD243; CLCS; Colchicin sensitivity; Doxorubicin resistance; GP170; Multidrug resistance protein 1 (MDR1); P-glycoprotein 1 (PGY1)

Database Links

Entrez Gene ID
5243
SwissProt
P08183

Additional Information

Clone
MDR1/8986R
Chromosome Location
7q21.12
Mol. Weight of Antigen
170kDa

Related Antibodies

View All ABCB1 Antibodies

Functions

  • Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218, PubMed:35970996). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218, PubMed:35970996).

Key References

  • Tedder, T.F., et al. 1995. FASEB J. 10: 866-873.
  • Lasky, L.A. 1995. Ann. Rev. Biochem. 64: 113-139.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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