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p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody [KIP1/769]

In Stock
Catalog Number Formulation Size Price
1027-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1027-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1027-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).

p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).

p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).

p27 Antibody in Flow Cytometry (FC)

p27 Antibody in Flow Cytometry (FC)

Flow Cytometry of human p27 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Mouse Monoclonal Antibody (KIP1/769).

p27 Antibody in Immunohistochemistry (IHC (P))

p27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded rat Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).

p27 Antibody in Immunofluorescence (IF)

p27 Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling p27 with p27 Mouse Monoclonal Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640

p27 Antibody in SDS-PAGE

p27 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified p27 Mouse Monoclonal Antibody (KIP1/769). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 /
Cellular Localization
Cytoplasm, Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
ZR75, MCF7, MDA-MB-231 or SK-BR-3 cells.
Immunogen
Recombinant full-length human CDKN1B protein
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, KIP1, MEN1B, MEN4

Database Links

Entrez Gene ID
1027
SwissProt
P46527

Additional Information

Clone
KIP1/769
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa

Related Antibodies

View All CDKN1B Antibodies

Functions

  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Key References

  • Fredersdorf S, et. al. Proc Natl Acad Sci 1997;94:6380-5.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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