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PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Antibody [PDCD1/7125]

In Stock
Catalog Number Formulation Size Price
5133-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5133-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5133-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with PD1 (CD279) Mouse Monoclonal Antibody (PDCD1/7125). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Antibody - Image 2

IHC analysis of FFPE human skin (RNA expression: 0.2 NX). Negative tissue control using PDCD1/7125 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

PD1 / CD279 / PDCD1 Antibody in SDS-PAGE

PD1 / CD279 / PDCD1 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified PD1 Mouse Monoclonal Antibody (PDCD1/7125). Confirmation of Purity and Integrity of Antibody.

PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Antibody in Western Blot (WB)

PDCD1 / PD1 / CD279 (Programmed Cell Death 1) Antibody in Western Blot (WB)

Western Blot Analysis of Human Tonsil and Brain tissue lysate using PD1 Mouse Monoclonal Antibody (PDCD1/7125).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Infectious Disease, PD-1 blockade immunotherapy
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
PDCD1
Positive Control
Human tonsil, lymph node or appendix.
Immunogen
Recombinant fragment (around aa1-200) of human PDCD1 protein (exact sequence is proprietary)
Alternate Names
Programmed cell death protein 1, CD279; hPD-1; hSLE1; PD1; PDCD1; Programmed Cell Death Protein 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2

Database Links

Entrez Gene ID
5133
SwissProt
Q15116

Additional Information

Clone
PDCD1/7125
Chromosome Location
2q37.3
Mol. Weight of Antigen
55kDa

Related Antibodies

View All PDCD1 Antibodies

Functions

  • Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
  • The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

Key References

  • Roncador, G., et al. Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. Haematologica. 2007 Aug;92(8):1059-66.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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