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PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody [31A3]

In Stock
Best Seller
Catalog Number Formulation Size Price
7345-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7345-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7345-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody

Formalin-fixed, paraffin-embedded human brain stained with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody - Image 2

Western blot of UchL1 (PGP9.5) in 1) human, 2) Mouse and 3) rat brain lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody - Image 3

Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody - Image 4

Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using PGP9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Dog, Human, Mouse, Pig, Rat
Research Areas
Neuroscience
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane
Gene Name
UCHL1
Positive Control
Human brain, T98G cells. Cerebellum.
Immunogen
Native UchL1 (PGP9.5) protein from brain
Alternate Names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1

Database Links

Entrez Gene ID
7345
SwissProt
P09936

Additional Information

Clone
31A3
Chromosome Location
4p13
Mol. Weight of Antigen
20-30kDa

Related Antibodies

View All UCHL1 Antibodies

Functions

  • Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865).

Key References

  • Day IN et. al. Biochem Society Trans 14:350-351 (1986)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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