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PHF10 (Transcription Factor) Antibody [PCRP-PHF10-2A10]

In Stock
Catalog Number Formulation Size Price
55274-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
55274-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
55274-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
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PHF10 (Transcription Factor) Antibody

Flow cytometric analysis of PFA-fixed HeLa cells. PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

PHF10 (Transcription Factor) Antibody - Image 2

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

PHF10 (Transcription Factor) Antibody - Image 3

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Mouse Monoclonal Antibody (PCRP-PHF10-2A10)

PHF10 (Transcription Factor) Antibody - Image 4

followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG2a /
Cellular Localization
Nucleus
Gene Name
PHF10
Positive Control
HeLa cells.
Immunogen
Recombinant full-length humanPHF10protein
Alternate Names
PHD finger protein 10, BRG1-associated factor 45a, XAP135, BAF45a; BRG1-associated factor 45a; FLJ10975; MGC111009; PHD finger protein 10; PHD finger protein 10, isoform a; PHD zinc finger protein XAP135; Phf10; XAP135

Database Links

Entrez Gene ID
55274
SwissProt
Q8WUB8

Additional Information

Clone
PCRP-PHF10-2A10
Chromosome Location
6q27
Mol. Weight of Antigen
56.05kDa

Related Antibodies

View All PHF10 Antibodies

Functions

  • Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Key References

  • Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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