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Recombinant PMEPA1 / TMEPAI (Tumor Suppressor Oncoprotein) Antibody [rPMEPA1/6422]

In Stock
Catalog Number Formulation Size Price
56937-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
56937-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
56937-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colon carcinoma stained with PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422).

Formalin-fixed, paraffin-embedded human prostate stained with PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422).

Formalin-fixed, paraffin-embedded human prostate stained with PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422).

SDS-PAGE Analysis of Purified PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified PMEPA1 Recombinant Mouse Monoclonal (rPMEPA1/6422). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

PMEPA1 (prostate transmembrane protein, androgen induced 1 is a 287 amino acid single-pass membrane protein that contains WW-binding motifs and localizes to the cell membrane. Expressed at high levels in prostate, kidney and ovary, PMEPA1 interacts with NEDD4 and may play a role in regulating AR (androgen receptor) levels, specifically in prostate cells. Down regulation of PMEPA1 is observed in prostate tumors, suggesting that PMEPA1 may exhibit activity as a tumor suppressor. Overexpression of this protein may play a role in multiple types of cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Early endosome membrane, Golgi apparatus membrane
Gene Name
PMEPA1
Positive Control
Human prostate, breast or renal cell carcinoma tissue (IHC).
Immunogen
Recombinant full-length human PMEPA1 protein
Alternate Names
Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein, PMEPA; PMEPA1; Prostate transmembrane protein, androgen induced 1; Solid tumor-associated 1 protein; STAG1; TMEPAI; Transmembrane prostate androgen-induced protein; Transmembrane, prostate androgen induced RNA

Database Links

Entrez Gene ID
56937
SwissProt
Q969W9

Additional Information

Clone
rPMEPA1/6422
Chromosome Location
20q13.31
Mol. Weight of Antigen
32kDa

Related Antibodies

View All PMEPA1 Antibodies

Functions

  • Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.

Key References

  • Xu, L.L., et al. 2000. Genomics 66: 257-263.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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