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Recombinant Prostate Specific Antigen (PSA) Antibody [rKLK3/6947]

In Stock
Catalog Number Formulation Size Price
354-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
354-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
354-MSM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb 1A7G6B6 for PSA tests.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm.
Gene Name
KLK3
Positive Control
PC12 cells. Normal prostate or prostate carcinoma tissues.
Immunogen
Recombinant fragment (around aa150-250) of human KLK3 (PSA) protein (exact sequence is proprietary)
Alternate Names
Prostate-specific antigen, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Database Links

Entrez Gene ID
354
SwissProt
P07288

Additional Information

Clone
rKLK3/6947
Chromosome Location
19q13.33
Mol. Weight of Antigen
33-34kDa

Related Antibodies

View All KLK3 Antibodies

Functions

  • Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Key References

  • ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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