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Prostate Specific Antigen (PSA) Antibody [SPM352]

In Stock
Catalog Number Formulation Size Price
354-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
354-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
354-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Prostate Specific Antigen (PSA) Antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with PSA Monoclonal Antibody (SPM352).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
KLK3
Positive Control
PC12 cells. Normal prostate or prostate carcinoma.
Immunogen
PSA from human sperm plasma
Alternate Names
Prostate-specific antigen, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Database Links

Entrez Gene ID
354
SwissProt
P07288

Additional Information

Clone
SPM352
Chromosome Location
19q13.33
Mol. Weight of Antigen
33-34kDa

Related Antibodies

View All KLK3 Antibodies

Functions

  • Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Key References

  • ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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