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Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody [rPU1/2146]

In Stock
Catalog Number Formulation Size Price
6688-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6688-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6688-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Recombinant PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology, Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
SPI1
Positive Control
Colon carcinoma., Hodgkin s Lymphoma, K562 or THP-1 cells. Lymph Node, Ramos, Spleen
Immunogen
Recombinant fragment (around aa 16-170) of human PU.1 protein (exact sequence is proprietary)
Alternate Names
Transcription factor PU.1, 31 kDa-transforming protein, Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Database Links

Entrez Gene ID
6688
SwissProt
P17947

Additional Information

Clone
rPU1/2146
Chromosome Location
11p11.2
Mol. Weight of Antigen
40kDa

Related Antibodies

View All SPI1 Antibodies

Functions

  • Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).

Key References

  • Loddenkemper C, et al. J Pathol. 2004; 202:60-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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