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Recombinant FOLH1 / PSMA (Prostate Epithelial Marker) Antibody [FOLH1/9735R]

In Stock
Catalog Number Formulation Size Price
2346-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2346-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2346-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
PSMA Antibody in Immunohistochemistry (IHC (P))

PSMA Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human prostate stained with Prostate Specific Membrane Antigen Recombinant Rabbit Monoclonal Antibody (FOLH1/9735R). Inset: PBS instead of primary antibody; secondary only negative control.

PSMA Antibody in SDS-PAGE

PSMA Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Prostate Specific Membrane Antigen Recombinant Rabbit Monoclonal Antibody (FOLH1/9735R). Confirmation of Purity and Integrity of Antibody.

PSMA Antibody in Western Blot (WB)

PSMA Antibody in Western Blot (WB)

Western Blot Analysis of LNCap cell lysate using FOLH1 Rabbit Recombinant Monoclonal Antibody (FOLH1/9735R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Prostate Cancer
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
FOLH1
Positive Control
LNCap or HepG2 cells. Prostate Carcinoma.
Immunogen
Recombinant human FOLH1 protein fragment (around aa1-200) (exact sequence is proprietary)
Alternate Names
Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, Cell growth-inhibiting gene 27 protein (GIG27); Folate hydrolase 1 (FOLH1); Folylpoly-gamma-glutamate carboxypeptidase (FGCP); Glutamate carboxylase II (GCPII); Glutamate carboxypeptidase II Membrane glutamate carboxypeptidase N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1 or NAALADase); Prostate-specific membrane antigen (PSM or PSMA); Pteroylpoly-gamma-glutamate carboxypeptidase

Database Links

Entrez Gene ID
2346
SwissProt
Q04609

Additional Information

Clone
FOLH1/9735R
Chromosome Location
11p11.12
Mol. Weight of Antigen
100kDa

Related Antibodies

View All FOLH1 Antibodies

Functions

  • Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.
  • Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Key References

  • Israeli, R.S., et al. 1993. Cancer Res. 53: 227-230.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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