Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant IDH1-R132G (Isocitrate Dehydrogenase) Antibody [IDH1.R132G/13054R]

In Stock
Catalog Number Formulation Size Price
3417-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3417-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3417-RBM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
IDH1-R132G Antibody in Immunohistochemistry (IHC (P))

IDH1-R132G Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human prostate cancer stained with IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R). Inset: PBS instead of primary antibody; secondary only negative control.

IDH1-R132G Antibody in SDS-PAGE

IDH1-R132G Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R). Confirmation of Purity and Integrity of Antibody.

IDH1-R132G (Isocitrate Dehydrogenase) Antibody in Western Blot (WB)

IDH1-R132G (Isocitrate Dehydrogenase) Antibody in Western Blot (WB)

Western Blot Analysis of human Kidney tissue lysate using IDH1-R132G Recombinant Rabbit Monoclonal Antibody (IDH1.R132G/13054R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The gene represented in this entry is involved in disease pathogenesis. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Infectious Disease, Nuclear Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Cytosol, Peroxisome
Gene Name
IDH1
Positive Control
Human breast, Kidney, colon or prostate carcinoma. HeLa, HepG2, HT29 or MCF-7 cells.
Immunogen
Recombinant fragment of human IDH1 protein with R132G (exact sequence is proprietary)
Alternate Names
Isocitrate dehydrogenase [NADP] cytoplasmic, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD

Database Links

Entrez Gene ID
3417
SwissProt
O75874

Additional Information

Clone
IDH1.R132G/13054R
Chromosome Location
2q33.3
Mol. Weight of Antigen
45-47kDa

Related Antibodies

View All IDH1 Antibodies

Functions

  • Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

Key References

  • Capper D, et al: Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol 2010 Jan;20(1):245-254

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant IDH1-R132G (Isocitrate Dehydrogenase) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales