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S100B (Astrocyte and Melanoma Marker) Antibody []

Name: S100B (Astrocyte and Melanoma Marker) Antibody
SKU: 6285-RBP5
Availability: InStock

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Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Polyclonal Antibody.

Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Rabbit Polyclonal Antibody.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Product Properties & Targets

Antibody Type

Rabbit Polyclonals

Host

Rabbit

Applications

Immunohistochemistry

Species Reactivity

Cow, Human, Mouse, Rat

Research Areas

Cardiovascular, Immunology, Neuroscience, Cytokine Signaling, Dendritic Cell Marker, Neural Stem Cells, Nuclear Marker, Signal Transduction

Isotype / Light Chain

IgG / Kappa

Cellular Localization

Cytoplasm, Nucleus

Gene Name

S100B

Positive Control

Brain, Melanoma or Schwanoma.

Immunogen

Recombinant full-length human S100B protein

Alternate Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, NEF; Protein S100-B; S-100 protein beta chain; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 protein beta chain; S100B; S100beta

Database Links

Entrez Gene ID

6285

SwissProt

P04271

Additional Information

Chromosome Location

21q22.3

Mol. Weight of Antigen

10-12kDa

Functions

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

Key References

Heizmann, C.W. 1988. Calcium-binding proteins of the EF-type. J. Cardiovasc. Pharmacol. 5: S30-S37.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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