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SAMD4B (Transcription Factor) Antibody [PCRP-SAMD4B-1H3]

In Stock
Catalog Number Formulation Size Price
55095-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
55095-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
55095-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
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SAMD4B (Transcription Factor) Antibody

Flow cytometric analysis of PFA-fixed HeLa cells. SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SAMD4B (Transcription Factor) Antibody - Image 2

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3)

SAMD4B (Transcription Factor) Antibody - Image 3

followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SAMD4B (Transcription Factor) Antibody - Image 4

SDS-PAGE Analysis SAMD4B Mouse Monoclonal Antibody (PCRP-SAMD4B-1H3). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2c /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
SAMD4B
Positive Control
HeLa cells.
Immunogen
Recombinant full-length humanSAMD4Bprotein
Alternate Names
Protein Smaug homolog 2, Sterile alpha motif domain-containing protein 4B, Sterile alpha motif domain containing 4B; hSmaug2; MGC99832; SMGB

Database Links

Entrez Gene ID
55095
SwissProt
Q5PRF9

Additional Information

Clone
PCRP-SAMD4B-1H3
Chromosome Location
19q13.2
Mol. Weight of Antigen
75.48kDa

Related Antibodies

View All SAMD4B Antibodies

Functions

  • Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A.

Key References

  • Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.
  • Luo N, et al.SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity. BMB Rep, 2010 May. PMID 20510020

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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