Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody [SDHB/3204]

In Stock
Catalog Number Formulation Size Price
6390-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6390-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6390-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with SDHB Mouse Monoclonal Antibody (SDHB/3204). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified SDHB Mouse Monoclonal Antibody (SDHB/3204). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. �Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. �Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST. �

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Colon Cancer, Mitochondria Marker
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Cytoplasm. Mitochondria.
Gene Name
SDHB
Positive Control
Jurkat or HepG2 cells. Human kidney or liver.
Immunogen
Recombinant human SDHB fragment around aa 165-273 (Exact sequence is proprietary)
Alternate Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, CWS2; DHSB; Iron-sulfur subunit of complex II; PGL4; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein

Database Links

Entrez Gene ID
6390
SwissProt
P21912

Additional Information

Clone
SDHB/3204
Chromosome Location
1p36.13
Mol. Weight of Antigen
32kDa

Related Antibodies

View All SDHB Antibodies

Functions

  • Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Key References

  • Allibhai, Z., et al. 2004. Malignant pheochromocytoma associated with germline mutation of the SDHB gene. J. Urol. 172: 1409-1410.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales