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SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody [SDHB/3745]

In Stock
Catalog Number Formulation Size Price
6390-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6390-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6390-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody

Formalin-fixed, paraffin-embedded human liver stained with SDHB Mouse Monoclonal Antibody (SDHB/3745). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified SDHB Mouse Monoclonal Antibody (SDHB/3745). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. �Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. �Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST. �

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Colon Cancer, Mitochondria Marker
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Cytoplasm. Mitochondria.
Gene Name
SDHB
Positive Control
Jurkat or HepG2 cells. Human kidney or liver.
Immunogen
Recombinant human SDHB fragment around aa 165-273 (Exact sequence is proprietary)
Alternate Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, CWS2; DHSB; Iron-sulfur subunit of complex II; PGL4; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein

Database Links

Entrez Gene ID
6390
SwissProt
P21912

Additional Information

Clone
SDHB/3745
Chromosome Location
1p36.13
Mol. Weight of Antigen
32kDa

Related Antibodies

View All SDHB Antibodies

Functions

  • Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Key References

  • Allibhai, Z., et al. 2004. Malignant pheochromocytoma associated with germline mutation of the SDHB gene. J. Urol. 172: 1409-1410.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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